Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Quality Biological Inc TE pH 7.4 100mL
TE is composed of buffering agent Tris and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together this Tris-EDTA based solution solubilizes DNA or RNA while protecting it from degradation. Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Bioworld TBE Buffer (10X), 4 L
Product DescriptionTBE (Tris-Borate-EDTA) has a liquid concentrate of 10X, where 1X contains 0.089M Tris, 0.089M Borate, and 0.002M EDTA. 1L of concentrate makes 10L of 1X buffer. Tris solutions are effective buffers for slightly basic conditions. Borate, a derivative of boric acid, is a weak proton donor but a Lewis acid that can accept an electron pair. EDTA is a chelator of divalent cations, mainly Mg2+. TBE is most commonly used for nucleic acid electrophoresis.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
Sigma Aldrich Fine Chemicals Biosciences mPAGER 8 Bis-Tris Precast
mPAGE 8% Bis-Tris Precast Gel 10x8 cmUnless otherwise stated in our catalog or other company documentation accompanying the product(s) our products are intended for research use only and are not to be used for any other purpose which includes but is not limited to unauthorized commercial uses in vitro diagnostic uses ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
eMolecules trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | 895245-31-5 | MFCD15071831 | 1g
J&W PharmLab LLC | trans-3,4-Bis-hydroxymethyl-pyrrolidine-1-carboxylic acid tert-butyl ester | 1g | 592901059 | 75R1156 | 97.000 | 895245-31-5 | MFCD15071831 | 231.292 | C11H21NO4
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eMolecules methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 502650-56-8 | | 1g
Pharmablock - Stock | methyl cis-3-(hydroxymethyl)cyclohexanecarboxylate | 1g | 534153256 | PBJL037 | | 502650-56-8 | | 172.224 | C9H16O3
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Sigma Aldrich Fine Chemicals Biosciences Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | 60-00-4 | MFCD00003541 | 1KG
Ethylenediaminetetraacetic acid ACS reagent, 99.4-100.6%, powder | Purity: 99.4-100.6% | Mol Wt: 292.24 | 60-00-4 | MFCD00003541 | 1KG
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 100G GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade , multicompendial
Rockland Immunochemicals TM Buffer (50 mM Tris Cl, pH 7.5, 10 mM Magnesium Sulfate) - MB-059
1X TM Buffer (50 mM Tris HCl, 10 mM Magnesium Sulfate at pH 7.5) is suitable for molecular biology assays.
Bioworld Tricine-SDS Sample Buffer, Reducing (2X), 50 mL
Product DescriptionTricine-SDS is a sample buffer under reducing conditions for protein analysis. It has a concentration of 2X and contains 900 mM Tris-HCl with a pH of 8.45, 8% SDS, 20% glycerol, 0.01% Coomassie blue, 0.01% phenol red, and 4% β-mercaptoethanol.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.